An integrated model for tissue engineered cartilage repair in vitro

doi:10.3969/j.issn.2095-4344.2014.27.011

Abstract

Abstract:

BACKGROUND: With the development of tissue engineering, autologous chondrocyte implantation is often used to repair cartilage defects. And poor integration is one of the common reasons that lead to failure repairing. Many models in vitro are used for related studies.
OBJECTIVE: To develop an interface integrated model of tissue engineered cartilage repair in vitro and to evaluate the effect.
METHODS: Cartilage integration model in vitro was established in pigs. Totally 21 cartilaginous rings were obtained and divided into agarose gel group (n=18) and control group (n=3). In agarose gel group, cartilage rings were covered with agarose gel. Chondrocytes were separated and implanted into the ring. The leakage of cells around the cartilage rings was observed. The sections were stained for histological observation at 1, 2, 4 weeks. The average area of neochondrocytes was measured and compared.
RESULTS AND CONCLUSION: The results from the control group were not processed, because there was no chondrocyte aggregate formation in the center of the explant ring due to earlier chondrocyte leakage outside the explant. While no chondrocytes were found outside the explant ring in the agarose gel group. Tissue sections of the agarose gel group were stained by hematoxylin and eosin, alcian blue, Safranin-O and collagen type II immunohistochemistry at 1, 2, 4 weeks. Neochondrocytes proliferated within cartilage ring, and produced extracellular matrix. After 2 weeks of incubation, these inserted chondrocytes were significantly increased. There was no statistically significant increment between 2 weeks and 4 weeks (P > 0.05), although the area was further increased by 4 weeks. This model provides a convenient simulation of the cartilage integration process in vitro and has a potential application in studies of cartilage integration and cartilage tissue engineering.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: tissue engineering, cartilage, chondrocytes, cell transplantation, cartilage, articular

CLC Number:

R318

Zhou Jian-xin, Gao Feng, Gui Jian-chao, Yin Zhao-wei, Yang Xiao-fei, Xu Yang, Lu Yi-ming, Li Yang, Jiang Yi-qiu. An integrated model for tissue engineered cartilage repair in vitro[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(27): 4324-4329.

Figures/Tables 3

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